August 18, 2022

# Calibration of Volumetric Apparatus

1. Objective:

To lay down a procedure for calibration of volumetric apparatus used in the laboratory.

• Scope:

This SOP is applicable for calibration of volumetric apparatus in Quality Control.

• Responsibilities:

Chemist QC /Officer                       : To follow the procedure

• Accountability:

Head – Quality Control :  For System Compliance.

• Procedure:
• Volumetric glassware shall be calibrated at 23°C to 27°C.
• The volumetric apparatus shall be calibrated once in a year. For calibration select randomly two glassware of a particular volume capacity. Serial number on the glassware shall be considered the identification of the glassware during calibration. Only class A grade glass ware shall be used in quality control laboratory.
• Clean the glassware for calibration to remove any grease, dirty etc. by keeping it immersed in chromic acid for 30 minutes.
• Rinse the glass ware under running tap water then rinse it with twice with water and dry it at 50°C for 120 minutes. Insure the glassware is completely dry.
• Remove the glassware from the oven and keep it in dessictors, insure the ambient temperature is 25°C±2°C.
• Arrange a water bath consisting of beaker, containing water.
• Maintain the temperature of water bath at 25°C±0.5°C, throughout the calibration.
• Place the apparatus to be calibrated on the balance and note the weight (A), remove the apparatus and transfer the water at 25°C. So that check the liquid in the apparatus mark label.
• Weigh the apparatus and note down the weight (B).
• Calculate the volume of glassware, using following formula.

(B-A)

=——————————

0.99602

Where 0.99602 is the density of water at 25°C.

• Calibration of Volumetric flask (IS 915:1957):
• Maintain the temperature of the water bath at  25°C±0.5°C throughout the calibration.
• Tare the volumetric flask and fill the water up to mark and weigh. Calculate the volume of flask using the formula given below:

Weight of water (g)

Volume =   ————————————-

Density of water

• Tolerance Limit: As given in annexure I.
• Calibration of Burette (IS 1997:1967):
• Maintain the temperature of the water bath at  25°C±0.5°C throughout the calibration.
• Fill the burette with water up to the zero point.
• Tare the beaker.
• Empty the burette up to 10.0 ml in the suitable beaker and weigh.
• Calculate the volume using the formula given below:

Weight of water (g)

Volume =    ————————————

Density of water

• Similarly weigh the water up to 20.0, 30.0, 40.0 & 50.0 ml as applicable and calculate the volume using the above given formula and record.
• Calibration of Bulb Pipette (IS 1117:1975):
• Maintain the temperature of the water bath at  25°C±0.5°C throughout the calibration..
• Tare a suitable beaker.
• Pipette the water up to the mark and empty the pipette in tarred beaker and weigh.
• Calculate the volume using the formula given below:

Weight of water (g)

Volume =———————————–

Density of water

Calibration of Graduated Pipette (IS 4162:1967):

Maintain the temperature of the water bath at 25°C±0.5°C throughout the calibration.

Tare a suitable beaker.

Weigh the volume of water at different graduation in suitable tarred beaker and record.

Calculate the volume using the formula given below:

Weight of water (g)

Volume =    ———————————–

Density of water

Calibration of Measuring Cylinders (ISO4788:2005)

Maintain the temperature of the water bath at 25°C±0.5°C throughout the calibration.

Tare the measuring cylinder and fill the water up to mark and weigh.

Calculate the volume using the formula given below:

Weight of water (g)

Volume =    ———————————–

Density of water

• Broken glass wares shall not be used in the laboratory.
• If any glassware breaks, then inform to Head Quality Control.
• Annexure(S):
• NA
• Reference (S) ,

IS 915:1957, IS 1997:1967, IS 1117:1975, IS 4162:1967, ISO4788:2005

• Glossary

SOP                :           Standard Operating Procedure

QA                   :           Quality Assurance

QC                  :           Quality Control

IS                     :           Indian Standard

ºC                    :           Degree Celsius

ml                    :           Milli Litre

ISO                  :           International Standard organization

• Revision History

#### Bhanu Pratap Singh

BHANU PRATAP SINGH IS EXPERIENCED IN PHARMACEUTICAL, AUTHOR AND FOUNDER OF PHARMACEUTICAL GUIDESLINE (WWW.PHARMAGUIDESLINE.COM), A WIDELY READ PHARMACEUTICAL BLOG SINCE 2019. EMAIL:- INFO@PHARMAGUIDESLINE.COM

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