Fluorescence-activated cell sorting (FACS) is a technique to purify specific cell populations based on phenotypes detected by flow cytometry. This method enables researchers to better understand the characteristics of a single cell population without the influence of other cells.
Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. It is a useful scientific instrument, as it provides fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest.
Fluorescence-activated cell sorting (FACS), sometimes called fluorescence-assisted cell sorting, is a specialized type of flow cytometry that uses fluorescent markers to target and isolate cell groups. This cell sorting technique is commonly used in hematopoiesis, oncology, and stem cell biology research. FACS technology separates cells based on cell surface markers. Antigenic ligands, such as proteins and carbohydrates, give each cell a unique surface phenotype, and specific antibodies associated with the cell surface antigens are then used to target cells with those antigens.
An antibody matched with an antigen on the surface of the targeted cell is labeled with a fluorescent molecule and mixed into the cell sample. One by one, the cells are passed in a continuous flow through a laser beam. Each cell scatters some of the laser light, and cells tagged with the identifying antibody emit a fluorescent light. Cells are deposited into containers based on their fluorescence or specific light scattering pattern Cells can be separated by fluorescence or by scattered light, either a forward (parallel) scatter that indicates cell size or a side (perpendicular) scatter that indicates granularity.