Flow cytometry(analysis)

Flow Cytometry is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.

Fluorescence

Flow cytometry is a widely used method for analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations, and analyzing cell size and volume.

Fluorescence activated cell sorting (FACS)

Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles.

In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer.

Fluorescence microscopy

Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include:Cell counting, Cell sorting, Determining cell characteristics and function, Detecting microorganisms, Biomarker detection, Protein engineering detection, Diagnosis of health disorders such as blood cancers.

A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties.

A flow cytometer tube with suction straw.

Fluorescence In Situ hybridization

Fluorescence Spectroscopy

Facs sorting

Epifluorescence microscopy

Bhanu Pratap Singh

BHANU PRATAP SINGH IS EXPERIENCED IN PHARMACEUTICAL, AUTHOR AND FOUNDER OF PHARMACEUTICAL GUIDESLINE (WWW.PHARMAGUIDESLINE.COM), A WIDELY READ PHARMACEUTICAL BLOG SINCE 2019. EMAIL:- INFO@PHARMAGUIDESLINE.COM

View all posts by Bhanu Pratap Singh →

Leave a Reply

Your email address will not be published. Required fields are marked *

error: Content is protected !!