Fluorescence microscopy

A fluorescence microscope is much the same as a conventional light microscope with added features to enhance its capabilities.  This fluorescent species in turn emits a lower energy light of a longer wavelength that produces the magnified image instead of the original light source.

Epifluorescence microscopy

A fluorescence microscope is much the same as a conventional light microscope with added features to enhance its capabilities.

  • The conventional microscope uses visible light (400-700 nanometers) to illuminate and produce a magnified image of a sample.
  • A fluorescence microscope, on the other hand, uses a much higher intensity light source which excites a fluorescent species in a sample of interest. This fluorescent species in turn emits a lower energy light of a longer wavelength that produces the magnified image instead of the original light source.
  • http://www.pharmaguidesline.com/facs-sorting/

Fluorescent microscopy is often used to image specific features of small specimens such as microbes. It is also used to visually enhance 3-D features at small scales. This can be accomplished by attaching fluorescent tags to anti-bodies that in turn attach to targeted features, or by staining in a less specific manner. When the reflected light and background fluorescence is filtered in this type of microscopy the targeted parts of a given sample can be imaged. This gives an investigator the ability to visualize desired organelles or unique surface features of a sample of interest. This is achieved by using powerful light sources, such as lasers, that can be focused to a pinpoint. This focusing is done repeatedly throughout one level of a specimen after another. Most often an image reconstruction program pieces the multi level image data together into a 3-D reconstruction of the targeted sample.

Flow cytometry(analysis)

Fluorescent Microscope Image.

Fluorescence Spectroscopy

Fluorescence In Situ hybridization

Fluorescence activated cell sorting (FACS)

Fluorescence

Bhanu Pratap Singh

BHANU PRATAP SINGH IS EXPERIENCED IN PHARMACEUTICAL, AUTHOR AND FOUNDER OF PHARMACEUTICAL GUIDESLINE (WWW.PHARMAGUIDESLINE.COM), A WIDELY READ PHARMACEUTICAL BLOG SINCE 2019. EMAIL:- INFO@PHARMAGUIDESLINE.COM

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