Standard Operation and Calibration of BOD Incubator

Operation and Calibration of BOD Incubator

1.0 OBJECTIVE

To lay down the procedure for the Operation and Calibration of BOD Incubator.

2.0       SCOPE

This procedure is applicable to operation & calibration of BOD Incubator used for fungal incubation in Microbiology laboratory of Quality control department .

Make / Model              : ABC

Instrument ID No        : XYZ

Operating range         : 20-25oC

Location                      : Incubator room

3.0 RESPONSIBILITY

Microbiologist

4.0 DISTRIBUTION

Master copy                :           Quality Assurance Department

Controlled Copy – 1     :           Quality Control Department

5.0 PROCEDURE 

5.1 Operation Procedure:

  • Ensure the instrument is clean and free from dust.
  • Insert the incubator plug into the main power socket. 
  • Press the power switch.
  • Switch ON the incubator by pressing the “MAINS” key, provided on the front panel of the machine. 
  • After pressing the “MAINS” key orange color light will glow which indicate the machine has started. 
  • The digital temperature controller of the incubator on the front of incubator, displays the set temperature in green colour and actual inside chamber temperature in red colour.
  • A “BLACK” key is provided on the front of incubator for “COOLING” purpose.
  • Inside the instrument chamber three trays are provided.
  • Load the stuff in the tray and close the door.
  • A Validation port is also provided in side of incubator for validation purpose.

5.2      Setting the Incubator Temperature:

  • The temperature controller has three SEL, andkeys.
  • Gently press the “SEL” key to save the change.
  • Operating range of this BOD incubator is 20-25oC and set the required temperature at  22.5oC by up “” and down “”key.
  • Set temperature starts displaying on the lower display.  

5.3 Calibration:

  • Calibration Frequency: Monthly
  • Follow the operating procedure for the operation.
  • The incubator thermometer will be calibrated using the calibrated thermometer.
  • Record the temperature 150C, 200C, 22.50C, 250C, 300C at the set temperature.
  • Record the results of calibration in the respective calibration record.Action to be taken in case of out of calibration.
  • After calibration affix the calibration label as per the label .

5.4 Acceptance Criteria:  

Temperature difference between the incubator thermometer and Calibrated thermometer shall be ± 1°C.

5.5 Maintenance :

  • Inform the Maintenance Department in case of malfunctioning is observed.
  • Stick the label of “UNDER MAINTENANCE”.

5.6 Precautions:

  • All the electric wires should be properly insulated. Temperature setting should not be disturbed once set.
  • Only authorized persons handle the instrument.

Bhanu Pratap Singh

BHANU PRATAP SINGH IS EXPERIENCED IN PHARMACEUTICAL, AUTHOR AND FOUNDER OF PHARMACEUTICAL GUIDESLINE (WWW.PHARMAGUIDESLINE.COM), A WIDELY READ PHARMACEUTICAL BLOG SINCE 2019. EMAIL:- INFO@PHARMAGUIDESLINE.COM

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