To lay down the procedure for cleaning or flushing of HPLC and UPLC system in Quality
This SOP is applicable for cleaning and flushing of HPLC and UPLC system used in Quality Control department .
Officer / Executive / Team leader – Quality Control shall be responsible to perform and maintaining relevant documentation.
Head QC/Designee shall be responsible for ensuring overall compliance of the SOP.
copy : Quality Assurance Department
Copy 1 : Quality Control Department
the exterior of the instrument with a dry lint free cloth or soft hair brush
and ensure that system shall be switch off during exterior cleaning.
start the cleaning or flushing, restart HPLC / UPLC system.
all suction filters and inline filters with boiled 10% nitric acid for about
15 minutes after that further sonicate with water than methanol.
HPLC / UPLC fill all the reservoirs with properly labeled respective
solvents. All lines shall dry and wet prime or purge all the solvent lines
before start the analysis. Wet prime or purge each pump to ensure that no air
is inadvertently introduced.
Shimadzu HPLC system clean rinsing bottle with water on daily basis.
the cleaning or flushing of HPLC / UPLC once in a month, preferably in the
first week of month with hot water for about 02 hours followed by methanol
for 30 minutes. During hot water flushing the column shall be replaced by
Standard Operating Procedure
High Pressure Liquid Chromatography
Ultra Performance Liquid Chromatography
Bhanu Pratap Singh
BHANU PRATAP SINGH IS EXPERIENCED IN PHARMACEUTICAL, AUTHOR AND FOUNDER OF PHARMACEUTICAL GUIDESLINE (WWW.PHARMAGUIDESLINE.COM), A WIDELY READ PHARMACEUTICAL BLOG SINCE 2019.